IphB KF1 from Comamonas testosteroni KF1 exhibits a ~4-fold higher catalytic efficiency with NADP+ than NAD+ in oxidizing 1,5-DCD.
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IphB KF1 from Comamonas testosteroni KF1 exhibits a ~4-fold higher catalytic efficiency with NADP+ than NAD+ in oxidizing 1,5-DCD.
GapC and GapD are substrate-specific auxiliary enzymes that contribute to redox balancing and metabolic module integration in Pseudomonas putida KT2440.
A thermophilic archaeon can directly degrade saturated and unsaturated fatty acids into methane, challenging the idea that methanogenesis requires syntrophic cooperation.
Many marine fungi, especially Pezizomycotina, possess a previously unrecognized DMSP lyase, DddF, enabling them to significantly contribute to DMS production in marine environments.
Low concentrations of cadmium (0.3-1.2 mg kg -1 ) have a negligible effect on methanotrophs in dryland farmland soils under field conditions.
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Programmable bioengineering and scalable bioprocessing enable fivefold increases in tailored rhamnolipid production from safe, non-pathogenic cell factories.
Liquid n-alkanes can be fully mineralized into carbon dioxide and water through microbial processes, enabling effective bioremediation of petroleum pollution.
Cryo-EM structures reveal a novel substrate channeling pathway in Paracoccus TMAO demethylase, linking TMAO degradation to formaldehyde transfer via a tunnel.
Engineered Escherichia coli with an orthogonal energy system enables growth and chemical production from CO₂ and methanol, doubling growth rate and high biomass yield.
A novel Rieske oxygenase system in Pseudomonas putida NCIMB 9866 enables successive oxidation of 2,4-xylenol’s ortho-methyl group into a valuable pharmaceutical precursor.
A conserved RH/QxxR motif enables widespread use of noncanonical redox cofactors in natural enzymes, with Bos taurus ALDH3a1 achieving unprecedented turnover with NMN+.
Balanced intracellular formate levels are essential for maintaining pH(i), CO2 homeostasis, and energy metabolism during mixed-acid fermentation in Escherichia coli, with disruptions causing growth defects.
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