A systematic interactome of Saccharomyces cerevisiae SET1C expands its functional landscape and identifies candidate regulatory connections.

Set1 interacts with over 100 proteins, expanding its known roles to include chromatin regulation, RNA processing, and nuclear-cytoplasmic transport in Saccharomyces cerevisiae.

• Why it matters: Understanding Set1’s diverse interactions is crucial because it influences key cellular processes like gene expression, DNA replication, and transposon activity, yet its full functional landscape remains unclear.
• What they did: Using yeast two-hybrid screens, the study mapped the interactome of Set1 and its subunits, identifying novel connections with RNA biogenesis factors, importins, and chromatin remodelers, including interactions with Snf2.
• The result: These findings reveal new regulatory mechanisms, such as Set1-dependent methylation of the Snf2 AT-hook domain, which may coordinate histone and non-histone methylation, broadening insights into epigenetic regulation.