Local translation of endosome-associated lc3b mRNA in axons contributes to endosomal clearance.

Endosome-associated lc3b mRNA is locally translated in axons, facilitating endosomal clearance through autophagy, with over 50% of endosomes showing LC3B colocalization.

• Why it matters: Understanding how neurons regulate subcellular protein synthesis is crucial for deciphering mechanisms of neuronal health and disease, especially regarding endosomal and autophagic processes.
• What they did: Using APEX-seq, the study identified numerous endosome-associated mRNAs, confirmed lc3b mRNA localization and translation in axons, and examined the effects of disrupting LC3B function on endosomal dynamics.
• The result: Local translation of lc3b mRNA at endosomes promotes their clearance, and impairments in this process lead to enlarged, dysfunctional endosomes, highlighting a vital link between local protein synthesis and neuronal autophagy.