From fragmented workflows to integrated pipelines: Bridging enzymatic DNA synthesis, assembly, and MutS-based error correction.

Integrating enzymatic DNA synthesis, assembly, and MutS-based error correction into a continuous pipeline enhances gene construction fidelity and scalability.

• Why it matters: Fragmented workflows limit the fidelity, throughput, and scalability of constructing large DNA sequences, impeding progress in synthetic biology. Bridging these stages is essential to overcome these constraints and enable efficient gene synthesis at the gene-to-pathway scale.
• What they did: The authors analyze error propagation during oligonucleotide synthesis and compare correction strategies, highlighting the superior efficiency of MutS-based mismatch depletion, and propose restructuring the pipeline to integrate synthesis, assembly, and error correction.
• The result: This integrated, error-aware workflow could enable high-fidelity, high-throughput, and scalable construction of gene- and pathway-scale DNA sequences, advancing synthetic biology capabilities.