Advanced Electron Microscopy Techniques and Applications

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CryoCodex: learning discrete structural representations for cryo-EM map post-processing
bioRxiv · 8 Oct 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.09.30.755675
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Genome-wide synthetic lethality screen of Bam complex-associated genes in Escherichia coli.
eLife · 8 Oct 2026 · Journal Article
Hundreds of synthetic-lethal interactions reveal that Bam complex activity in Escherichia coli is affected by outer membrane lipid composition and peptidoglycan synthesis genes.
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https://jclub.com.au/a/42848444
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In situ cryo-electron tomography of vaccinia virus exit from infected cells
bioRxiv · 6 Oct 2026 · Preprint
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https://jclub.com.au/a/10.1101/2025.08.20.671218
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Time-resolved functional rotation crystallography probes protein dynamics during enzymatic catalysis
bioRxiv · 3 Oct 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.04.24.718481
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cryoAgent: An agentic workflow for robust and adaptive end-to-end cryo-EM image processing
bioRxiv · 29 Sept 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.04.16.718662
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ProDive: detecting cross-family local conservation
bioRxiv · 28 Sept 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.04.02.715841
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Cryo-EM structures of CCHFV polymerase reveal a stepwise initiation stabilization pathway and a dual-site inhibition mechanism.
Cell Reports · 22 Sept 2026 · Journal Article
Cryo-EM structures reveal a stepwise stabilization pathway and dual-site inhibition mechanism of the CCHFV polymerase, offering new insights into viral replication control.
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https://jclub.com.au/a/42696455
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FoldaVirus, a knowledge-based icosahedral capsid prediction tool using AlphaFold.
Proceedings of the National Academy of Sciences · 22 Sept 2026 · Journal Article
FoldaVirus predicts complete icosahedral viral capsids up to T=9 symmetry by integrating AlphaFold structures with known viral architectures.
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https://jclub.com.au/a/42752236
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Integrative structure of norovirus NS3 suggests a role in RNA transport.
Nature Communications · 21 Sept 2026 · Journal Article
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https://jclub.com.au/a/42901927
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A Robust Hierarchical Linear Model for Cryo-EM Map Analysis
bioRxiv · 10 Sept 2026 · Preprint
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https://jclub.com.au/a/10.1101/2025.07.10.664269
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MemBrain v2: an end-to-end tool for the analysis of membranes in cryo-electron tomography.
Nature Methods · 8 Sept 2026 · Journal Article
MemBrain v2 achieves highly generalizable membrane segmentation across diverse cryo-electron tomograms using deep learning, addressing a key bottleneck in membrane analysis.
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https://jclub.com.au/a/42711494
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In extracto cryo-EM reveals eEF2 as a major hibernation factor on 60S and 80S particles.
eLife · 3 Sept 2026 · Journal Article
eEF2 is the dominant hibernation factor, binding to over 95% of 80S ribosomes and 60S subunits in mammalian cells, revealing a key role in cellular stress responses.
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DAQplugin: Interactive Deep Learning-Based Validation of Cryo-EM Protein Models in ChimeraX
bioRxiv · 3 Sept 2026 · Preprint
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Structural basis of transcription of the hachimoji eight-letter alphabet by E. coli RNA polymerase.
Nature Communications · 2 Sept 2026 · Journal Article
E. coli RNA polymerase can efficiently transcribe an eight-letter genetic alphabet, including two orthogonal unnatural base pairs, with high fidelity and structural compatibility.
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https://jclub.com.au/a/42686745
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Structural basis for co-translational assembly of homo-oligomeric proteins in cis and in trans
bioRxiv · 2 Sept 2026 · Preprint
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https://jclub.com.au/a/10.1101/2025.11.12.687954
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Author Correction: In situ cryo-ET defines the ultrastructure of ER exit sites in human cells.
Nature Cell Biology · 1 Sept 2026 · Published Erratum
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Meso2EM: a cross-scale CLEM workflow linking mesoscale functional imaging to targeted electron microscopy
bioRxiv · 1 Sept 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.08.25.746890
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Electron diffraction captures high-resolution structures from in vivo protein nanocrystals of Bacillus thuringiensis
bioRxiv · 28 Aug 2026 · Preprint
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https://jclub.com.au/a/10.1101/2025.06.24.661318
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OMIO: A policy-driven Python library for reproducible microscopy image I/O
bioRxiv · 25 Aug 2026 · Preprint
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