Advanced Fluorescence Microscopy Techniques

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HDCluster: High-Degree Graph Clustering for Robust Analysis of Single Molecule Localization Microscopy
bioRxiv · 17 Oct 2026 · Preprint
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https://jclub.com.au/a/10.1101/2025.10.23.684134
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Recent developments of fluorescent proteins and biosensors driven by cross-disciplinary insights.
Nature Methods · 16 Oct 2026 · Review
Recent advances in fluorescent proteins and biosensors have significantly improved photostability, wavelength range, and multiplexing capabilities, exemplified by mScarlet.
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Development of a genetically encoded fluorescent indicator for monitoring GPR52 activation
bioRxiv · 14 Oct 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.03.11.711017
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A StayGold-based calcium ion indicator
bioRxiv · 12 Oct 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.03.06.710044
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Functional and structural profiling of circulation via genetically encoded modular fluorescent probes.
bioRxiv · 12 Oct 2026 · Preprint
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https://jclub.com.au/a/10.1101/2025.03.18.643859
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Tracking Without Linking or Localization: Single-Photon Tracking
bioRxiv · 10 Oct 2026 · Preprint
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https://jclub.com.au/a/10.1101/2025.01.10.632389
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Nuclear-BRITE: an enhanced MS2 system for live-cell imaging of nuclear RNAs
bioRxiv · 9 Oct 2026 · Preprint
Nuclear-BRITE enables fast, single-molecule live-cell imaging of nuclear RNAs with reduced background noise, surpassing previous MS2 system limitations.
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https://jclub.com.au/a/10.64898/2026.07.07.736975
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Design of Orthogonal Far-Red, Orange and Green Fluorophore-binding Proteins for Multiplex Imaging
bioRxiv · 9 Oct 2026 · Preprint
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An integrated open-source acquisition and analysis ecosystem for high-throughput single-molecule imaging.
Nature Methods · 9 Oct 2026 · Letter
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Streamlined Production of Recombinant Adeno-Associated Viruses Using Gateway Cloning Technology for Neural Circuit All-Optical Interrogation
bioRxiv · 8 Oct 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.09.22.752944
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Mapping Mesoscale Cardiac Architecture with Axially Swept Dithered Light-Sheet Microscopy
bioRxiv · 8 Oct 2026 · Preprint
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https://jclub.com.au/a/10.1101/2025.10.13.682248
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High-throughput serial two-photon tomography with BakingTray and BrainSaw
bioRxiv · 7 Oct 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.09.28.748989
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Fluorescent nanodiamond phantoms for spatial resolution benchmarking in two- and three-photon microscopy
bioRxiv · 6 Oct 2026 · Preprint
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Fluorescent proteins misreport ribosome abundance under translation inhibition
bioRxiv · 5 Oct 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.09.17.752513
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Measuring protein conformational changes with genetically encoded bipyridylalanine and transition-metal ion FRET
bioRxiv · 2 Oct 2026 · Preprint
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SIGMA: SIgnaling via Golgi Morphology Analysis for pathway-state classification
bioRxiv · 2 Oct 2026 · Preprint
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Chromosome-scale assembly of yeast genomes using enzyme-free, dense optical genome mapping.
Nature Communications · 1 Oct 2026 · Journal Article
Dense optical genome mapping achieves chromosome-scale assembly of yeast genomes, revealing structural variations and repetitive regions up to 500 kbp in size.
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Multiplexed imaging.
Nature Chemical Biology · 1 Oct 2026 · Journal Article
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SPIFFI enables single-shot super-resolution and multidimensional imaging.
Nature Methods · 1 Oct 2026 · Journal Article
SPIFFI achieves instant super-resolution and six-dimensional imaging from a single exposure, enabling real-time biological observations beyond the diffraction limit.
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https://jclub.com.au/a/42675117
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Design and Evolution of an Orthogonal HaloTag for Multiplexed Labeling in Cells
bioRxiv · 1 Oct 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.05.14.725131
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Polarized detection sharpens optical live-cell fluorescence imaging.
Nature Methods · 1 Oct 2026 · Journal Article
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CLOK: a chemigenetic multicolor labeling system to visualize neuronal birthdate and circuit integration.
Nature Neuroscience · 30 Sept 2026 · Journal Article
CLOK enables precise multicolor labeling of neurons by birthdate, revealing distinct maturation trajectories in zebrafish visual and motor systems.
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Fixing cells with light and DNA-binding fluorophores
bioRxiv · 26 Sept 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.03.27.714905
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Constitutive, endogenous, fluorescent membrane reporters for dynamic cell cycle analysis in Bacillus subtilis.
Applied and environmental microbiology · 23 Sept 2026 · Journal Article
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A Bottom-Up Platform for Quantitative Single-ParticleTracking Through Bacterial Biofilm Mimics
bioRxiv · 19 Sept 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.07.02.736016
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An All-Optical Approach to Probe Chloride Transport with a Bright ChlorON
bioRxiv · 17 Sept 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.03.09.710669
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Molecular identification of wide-field amacrine cells in mouse retina that encode stimulus orientation
bioRxiv · 17 Sept 2026 · Preprint
Bhlhe22 and Kappa Opioid Receptor-expressing wide-field amacrine cells in mouse retina encode stimulus orientation with Ca2+ signals aligned to dendritic structure.
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https://jclub.com.au/a/10.1101/2023.12.28.573580
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QuantiTrack: A unified software to study protein dynamics in living cells
bioRxiv · 15 Sept 2026 · Preprint
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https://jclub.com.au/a/10.64898/2026.02.19.706877
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Imaging cellular activity across all organs reveals body-wide circuits.
Nature · 9 Sept 2026 · Journal Article
WHOLISTIC imaging reveals body-wide cellular activity and novel physiological responses across all organs in vertebrates.
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Rapid robust high-fidelity 3D neuronal extraction from multiview calcium imaging datasets.
Nature Methods · 7 Sept 2026 · Journal Article
DeepWonder3D achieves high-fidelity 3D neuronal extraction from large-scale calcium imaging datasets, reducing computational costs tenfold and processing tens of thousands of neurons in hours.
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