The Vizitig pangenome and pantranscriptome explorer.

Vizitig is the first platform to enable real-time exploration of DNA and RNA de Bruijn graphs across many samples, scaling from virus genomes to human pangenomes with up to 20 haplotypes.

• Why it matters: Understanding complex genomic variation and transcript fusion events requires tools that can handle large, diverse datasets interactively. Existing methods lack integrated visualization and flexible querying for such extensive data.
• What they did: The platform constructs compacted colored de Bruijn graphs from raw sequencing reads and reference sequences, offering an interactive web interface with a human-readable query language and on-demand subgraph loading, demonstrated through case studies in pantranscriptomics and pangenomics.
• The result: Vizitig enables fast, reproducible analysis of large genomic datasets, recovering fusion transcript breakpoints and exploring sequence variations across multiple species, all on a standard laptop.