G4All: a database of experimentally confirmed G-quadruplex-forming sequences.

G4All provides the first comprehensive, curated database of experimentally validated G-quadruplex-forming DNA and RNA sequences with negative controls.

• Why it matters: A centralized repository of validated G4 sequences is essential to improve reproducibility, enable rigorous comparative analyses, and advance therapeutic and biotechnological research.
• What they did: The database consolidates sequences validated by methods like circular dichroism, NMR, and UV spectroscopy, with annotations on topology, stability, and experimental conditions, covering around 100 mM potassium and near neutral pH.
• The result: G4All facilitates fundamental G4 biophysics studies, aptamer design, and prediction algorithm benchmarking, supporting community-driven expansion and standardization in G4 research.