High-Dimensional Spectral Flow Cytometry Panel for Immunophenotypic Characterization of Fixed Whole Blood Samples.

A 36-marker lymphoid and 35-marker myeloid spectral flow cytometry panel reliably characterizes immune populations in fixed whole blood samples, maintaining stability over 2.5 years.

• Why it matters: This approach addresses logistical challenges in immune profiling by eliminating the need for immediate PBMC isolation and cryopreservation, facilitating clinical research in resource-limited settings.
• What they did: Researchers developed and optimized spectral flow cytometry panels compatible with fixed blood, focusing on antibody clone compatibility and chemokine receptor detection, and validated performance against fresh samples.
• The result: The panels consistently identified major immune lineages in fixed blood, with profiles remaining stable over long-term storage, supporting their use in longitudinal immune monitoring.