Genomic characterization and phage cocktail suppression of a multidrug-resistant Vibrio sinaloensis isolate from diseased shrimp.

A multidrug-resistant Vibrio sinaloensis strain from diseased shrimp was effectively suppressed in vitro using a novel phage cocktail, reducing resistance development.

• Why it matters: Vibrio sinaloensis is linked to disease in aquaculture, and its multidrug resistance complicates management; understanding its genome and alternative control methods is critical for sustainable practices.
• What they did: Researchers sequenced the genome of strain ZZ006, identified three complementary bacteriophages, and tested their combined ability to inhibit bacterial growth and prevent resistance in vitro.
• The result: The phage cocktail suppressed ZZ006 growth and limited resistant mutants, revealing mutations in pilus- and pseudopilin-related genes, supporting phage therapy as a potential biocontrol strategy.