De novo protein design creates artificial metalloenzymes that achieve highly enantioselective photocatalysis with racemic metal complexes.
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De novo protein design creates artificial metalloenzymes that achieve highly enantioselective photocatalysis with racemic metal complexes.
Twenty noncanonical cytochrome P450 families lacking a conserved cysteine ligand are identified in diverse microbial genomes, including the first native selenocysteine-ligated CYP.
Glutaredoxins in plants have diversified into over 30 isoforms across four classes, with class III GRXs evolving plant-specific features that enable functional diversification.
Excised leaf samples in Arabidopsis show more photosystem II damage and less thylakoid structural change after high-light treatment than intact plants, highlighting the importance of sample integrity.
Mutational analysis reveals key base pair requirements for NrdR binding in prokaryotic genomes, enabling precise identification of regulatory sites.
A conserved RH/QxxR motif enables widespread use of noncanonical redox cofactors in natural enzymes, with Bos taurus ALDH3a1 achieving unprecedented turnover with NMN+.
Loss of PRDX1 enhances DNA damage response inhibition, revealing a critical iron-dependent damage axis in genome stability regulation.
Hyperthermophilic archaeal nitrogenase from Methanocaldococcus infernus exhibits the highest known activity and thermostability, with a melting temperature near water's boiling point.
Pyridoxal 5'-phosphate (PLP) acts as a photoenzymatic cofactor enabling asymmetric radical-radical cross-couplings, expanding enzymatic bond formation capabilities.
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