Multi-organelle signatures map cell-state diversity and metabolic adaptation in tissues.

Multi-organelle signatures identified distinct cell states and metabolic adaptations in liver and pancreas tissues, revealing complex intra-zonal heterogeneity and stress responses.

• Why it matters: Understanding cell-state diversity is crucial for tissue repair, adaptability, and disease resilience, but current methods overlook organelle structure, a key metabolic indicator.
• What they did: Using spatial Organellomics (sOrganellomics), an imaging workflow combining automated segmentation and machine learning, researchers mapped multi-organelle signatures across tissues, analyzing over 100,000 cells in vivo.
• The result: The approach uncovered that hepatocyte communities are intermixed within zones and respond dynamically to nutritional stress, linking organelle architecture to tissue adaptation and metabolic states.