Dual regulation of chemical stress-induced DDI2/3 expression by a transcription factor Fzf1 and nucleosome in Saccharomyces cerevisiae

Fzf1 regulates DDI2/3 expression in Saccharomyces cerevisiae through both transcription activation and chromatin remodeling, with CY treatment reducing nucleosome occupancy at the URS.

• Why it matters: Understanding how Fzf1 controls gene responses to chemical stress can reveal mechanisms of gene regulation under stress conditions, which are not fully explained by transcription factor binding alone.
• What they did: The study used promoter deletions, histone depletion experiments, and genome-wide MNase-seq analysis to investigate Fzf1's role in DDI2/3 regulation, focusing on the URS region and nucleosome dynamics.
• The result: Fzf1 acts as a dual regulator by binding CS2 to activate transcription and facilitating chromatin remodeling at the URS, enabling higher induction of DDI2/3 by CY and MMS, highlighting its potential as a pioneer transcription factor.