Delineating plant responses to the 3',5'- and 2',3'-cAMP isomers.

Both 3',5'- and 2',3'-cAMP isomers induce similar proteome changes in Arabidopsis thaliana, with 3',5'-cAMP showing specific enrichment in protein transport and localization.

• Why it matters: Understanding isomer-specific responses in plants is crucial for deciphering their physiological roles and signaling mechanisms, which remain partially unresolved.
• What they did: A comparative proteomics approach was used to analyze Arabidopsis exposed to 200 nM of each isomer, revealing shared effects on RNA processing proteins and distinct regulation of specific pathways.
• The result: Both isomers equally mitigate H2O2-induced ion fluxes, but CNGC2 and CNGC18 channels show strict selectivity for 3',5'-cAMP, suggesting in vivo gating depends on catalytically active adenylate cyclases.