Identification of Molecular Pathways Underlying Mn(II) Oxidation in Pseudomonas resinovorans.

Disruption of pilC, algR, and hk05390 genes impairs Mn(II) oxidation in Pseudomonas resinovorans MOB-513 by reducing biofilm formation and c-di-GMP levels.

• Why it matters: Understanding the molecular pathways of Mn(II) oxidation is crucial for improving biological manganese removal processes, yet specific components remain unidentified.
• What they did: Researchers used transposon mutagenesis to identify mutants with altered Mn(II) oxidation, focusing on genes linked to biofilm formation, c-di-GMP regulation, and Tfp production, and analyzed their effects on enzyme activity and signaling pathways.
• The result: Findings reveal two c-di-GMP-dependent regulators, including RR05389, modulate Mn(II) oxidation, providing new targets for optimizing manganese bioremediation strategies.