A high-confidence inventory of the proteins in the mitochondrion-related organelle of Giardia.

Giardia's mitochondrion-related organelle proteome includes 59 proteins, with 27 newly identified and 34 of unknown function, revealing new insights into its biology.

• Why it matters: Understanding Giardia's MRO proteins is crucial because the parasite lacks mitochondrial DNA and oxidative phosphorylation enzymes, yet retains these organelles, which are key to its survival and pathogenicity.
• What they did: The study used large-scale culture, density gradient fractionation, affinity purification, DIA-MS, protein-correlation profiling, and confocal microscopy to generate the Giardia MitoCarta (GlMitoCarta) inventory of 59 proteins.
• The result: The GlMitoCarta resource enables systematic studies of MRO function and Giardia pathogenesis, with findings such as MROX6 and MROX13 interactions and the identification of MOMTiP-4 as a likely inner membrane transporter.