Coolsecture: an easy-to-use and improved framework for cross-species Hi-C contact map comparison.

Coolsecture provides a streamlined, reproducible framework for cross-species Hi-C contact map comparison, integrating multiple formats and diagnostic tools.

• Why it matters: Comparing Hi-C contact maps across species is difficult due to reliance on multiple scripts, heterogeneous formats, and limited diagnostic support, hindering accurate analysis.
• What they did: The authors developed coolsecture, a Python 3 command-line toolkit that combines contact-matrix and synteny formats, bidirectional lift-over, reciprocal assessment, multi-resolution comparison, and visualization, with source code and workflows freely available.
• The result: Coolsecture enables more efficient and consistent cross-species Hi-C comparisons, facilitating improved analysis and reproducibility in comparative genomics studies.