DFCP1-containing ER microdomains mediate lysosomal membrane repair.

PI3P-containing ER microdomains, mediated by DFCP1, are essential for lysosomal membrane repair in response to damage.

• Why it matters: Understanding the mechanisms of lysosomal membrane repair is crucial for maintaining cellular homeostasis and combating damage from stressors like pathogens.
• What they did: The study used cellular models to show that PIK3C3/VPS34 generates PI3P on ER domains near lysosomes, recruiting DFCP1, which then promotes lipid transfer and lysosomal sealing.
• The result: DFCP1's ATPase activity is vital for lysosomal repair, and its absence reduces cellular resistance to vacuolar damage, highlighting its role in lysosomal integrity maintenance.