Integrating multi-omics to reveal the regulatory mechanisms of microfilament cytoskeleton in programmed cell death during seed cryopreservation.

Microfilament cytoskeleton modulates programmed cell death during seed cryopreservation, with stabilizers increasing seed viability by up to 20% in Paeonia lactiflora seeds.

• Why it matters: Understanding the molecular mechanisms of PCD in seed cryopreservation is crucial for improving seed storage and conservation strategies, addressing a key gap in plant biotechnology.
• What they did: Researchers integrated physiological, transcriptomic, and proteomic analyses of two seed types, using stabilizers and depolymerizers of microfilaments to explore their role in PCD regulation.
• The result: Findings reveal that microfilament dynamics influence PCD via pathways like protein processing in the endoplasmic reticulum and glycerolipid metabolism, with HSP and ALDH proteins as potential targets to enhance seed viability.