Yeast Strain Development and Process Intensification in High-Gravity Fermentation.

Yeast improvement strategies and process innovations support high- and very-high-gravity fermentation as a promising platform for bioproduct manufacturing.

• Why it matters: Increasing substrate loading and product titers in HG/VHG fermentation can enhance efficiency and reduce resource use, but face constraints like stress and transfer limitations.
• What they did: The review covers strategies such as adaptive evolution, mutagenesis, genome editing, and process developments like no-cook SLSF and in situ recovery, applied across various industries.
• The result: Current evidence indicates HG/VHG fermentation is a valuable process platform, with performance and sustainability influenced by feedstock, conditions, and outcome reporting methods.