Constitutive transcriptomic reprogramming underlies differential resistance of Flammulina velutipes to Pseudomonas tolaasii.

Constitutive up-regulation of 634 genes linked to redox, cell wall, and oxidoreductase functions underpins resistance in Flammulina velutipes to Pseudomonas tolaasii.

• Why it matters: Understanding the molecular basis of strain-dependent resistance is crucial for developing disease-resistant cultivars, as current knowledge about early mycelial defense mechanisms remains limited.
• What they did: The study compared resistant (S2595) and susceptible (S2506) strains using dual-culture assays, SEM, and transcriptome profiling with three biological replicates, revealing that resistance correlates with constitutive gene expression differences.
• The result: Findings suggest resistance is associated with a pre-established defense homeostasis rather than pathogen-induced transcriptional changes, providing candidate markers for breeding resilient F. velutipes cultivars.