Myosin1D directs circumferential F-actin flow to define cell chirality.

Myo1D directs clockwise circumferential F-actin flow in Drosophila macrophages, contributing to cell chirality and asymmetric morphogenesis.

• Why it matters: Understanding how intrinsic cell chirality forms is crucial for deciphering left-right asymmetry in development, yet its molecular mechanisms remain unclear.
• What they did: The study examined Myo1D, Myo1C, and Myo2 in Drosophila macrophages, using in vitro motility assays to observe F-actin flow patterns and organization.
• The result: Myo1D triggers self-organized clockwise F-actin rings, suggesting it assembles polarized actin structures, which, along with Myo2, underlie cell and organ chirality.