Multi-omics integration revealed the molecular responses and key candidate genes underlying cold tolerance in Elymus nutans.

Multi-omics analysis identified key genes EnP5CS2 and EnMDHAR4 that underpin cold tolerance in Elymus nutans, revealing a complex two-phase stress response.

• Why it matters: Understanding the molecular mechanisms of cold tolerance in Elymus nutans is crucial for developing climate-resilient crops and for conserving alpine plant species facing temperature extremes.
• What they did: Researchers integrated physiological, transcriptomic, and proteomic data from cold-stressed plants, uncovering distinct early and late response phases, and pinpointing candidate genes through network analysis and heterologous yeast expression.
• The result: Findings highlight resource reallocation strategies, such as downregulation of photosynthesis and activation of protective pathways, with candidate genes offering promising targets for breeding cold-tolerant forage and crops.