Integrated in vitro multi-endpoint framework for assessing 6PPD-Quinone cytotoxicity in fish and mammalian cell lines.

Fish-derived cell lines (PCK and RTG-2) show greater susceptibility to 6PPD-Q toxicity than mammalian cell lines, with pNPA values indicating higher phenotypic perturbations.

• Why it matters: Understanding the differential toxicity of 6PPD-Q across species is crucial for ecological risk assessment and regulatory decisions, yet comparative cellular effects remain underexplored.
• What they did: The study conducted integrated cellular and phenotypic profiling on five cell lines using assays such as viability, apoptosis, and cell cycle analysis, combined with scoring frameworks pNPA and RBIF to quantify toxicity.
• The result: Fish-derived cells exhibited higher toxicity indicators, suggesting species-specific cellular responses, and the framework enables objective comparison of phenotypic perturbations, although findings are limited to in vitro conditions.